Volume 70, Issue 1 p. 61-73
ORIGINAL ARTICLE

Genotypic and phenotypic characterization of Phytophthora infestans populations on Java, Indonesia

Sandesh Dangi

Sandesh Dangi

Department of Entomology, Plant Pathology and Nematology, University of Idaho, Aberdeen Research and Extension Center, Aberdeen, ID, USA

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Phillip Wharton

Corresponding Author

Phillip Wharton

Department of Entomology, Plant Pathology and Nematology, University of Idaho, Aberdeen Research and Extension Center, Aberdeen, ID, USA

Correspondence

Phillip Wharton, Department of Entomology, Plant Pathology and Nematology, University of Idaho, Aberdeen Research and ExtensionCenter, Aberdeen, Idaho 83210, USA.

Email: [email protected]

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Alberta D. Ambarwati

Alberta D. Ambarwati

Plant Molecular Biology Division, Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Ministry of Agriculture, Bogor, Indonesia

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Tri Joko Santoso

Tri Joko Santoso

Plant Molecular Biology Division, Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Ministry of Agriculture, Bogor, Indonesia

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Kusmana

Kusmana

Plant Molecular Biology Division, Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Ministry of Agriculture, Bogor, Indonesia

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Ineu Sulastrini

Ineu Sulastrini

Plant Molecular Biology Division, Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Ministry of Agriculture, Bogor, Indonesia

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John Medendorp

John Medendorp

Department of Plant, Soil and Microbial Science, Michigan State University, East Lansing, MI, USA

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Karen Hokanson

Karen Hokanson

Department of Horticultural Sciences, University of Minnesota, St Paul, MN, USA

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David Douches

David Douches

Department of Plant, Soil and Microbial Science, Michigan State University, East Lansing, MI, USA

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First published: 05 September 2020
Citations: 5

Abstract

Phytophthora infestans is endemic to Indonesia and can infect potato crops at any stage in the growing season. Little is known about P. infestans populations in Indonesia. The objectives of this study were first to identify the genotypes causing late blight in the main potato-growing regions on Java in Indonesia, and secondly to examine genotypic diversity in the P. infestans populations in those regions. Samples were collected on FTA cards (n = 140) or in tubers (n = 6) from 15 locations in nine regencies over four years (2016–2019). Microsatellite analysis revealed that late blight outbreaks in these regencies were caused by EU_2_A1 and other genotypes that are unique to Indonesia. Eighty percent of the samples that amplified with CAPS markers were the A1 mating type. Cultures of six isolates were determined to be the A1 mating type based on the pairing test, and of these, two isolates were intermediate and four were sensitive to metalaxyl-M (mefenoxam). The mode of reproduction of the P. infestans population on Java, Indonesia, was found to be clonal. However, as the sample size in this study was small, more isolates need to be tested to confirm this. Microsatellite analysis revealed that 90% of Indonesian samples had trisomic loci. A high number of multilocus genotypes (MLGs) were found in all nine regencies (131 MLGs out of 146 samples). Results indicate that there is ongoing polyploidization in these populations due to a high mutation rate and no selection pressure from the susceptible potato hosts that are being grown in Indonesia.

DATA AVAILABILITY STATEMENT

The data that support the findings of this study are available from the corresponding author upon reasonable request.